FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462205652
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG26069600.5640259890201429TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA4101100.0106.964541
TCGGAAG4326700.0101.530582
CGGAAGA4530050.096.963713
AGAGCAC7175400.061.4826627
AGCACAC7459000.059.095469
GAGCACA7543700.058.4799848
GAAGAGC7713850.057.3281755
AAGAGCA10238450.043.39896
GGAAGAG11387350.039.1858524
TATGCCG3482950.025.65931945-49
CGTATGC3596000.024.69421845-49
CTCGTAT3352000.024.58836640-44
TCTCGTA3413800.024.13687340-44
ATGCCGT3761250.023.71064645-49
GCCGTCT3831450.023.02429250-54
TGCCGTC3805700.022.98002845-49
TCGTATG3522750.022.96939340-44
ATCTCGT3653550.022.60734440-44
ACTCCGG3977950.022.49707830-34
CCGGAGA3942050.022.2680230-34
Produced by FastQC (version 0.11.5)