FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462205652
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC28478240.6161378571805088Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG4055950.0108.985919
GAGCGTC4445000.099.44698
ATCGGAA4582500.097.540091
TCGGAAG4798300.092.968172
AGAGCGT4796500.092.3698357
CGGAAGA5016750.088.8442843
AAGAGCG5259200.084.621746
GAAGAGC8179850.054.8038645
GGAAGAG11945650.037.88434
CGCCGTA3439900.026.01490655-59
TCGCCGT3603000.024.81835455-59
GCCGTAT3676450.024.42938855-59
GTCGCCG3782900.023.6277455-59
CCGTATC3807450.023.195955-59
TGGTCGC3887550.023.1434850-54
CGTATCA3889950.022.9495460-64
GCGTCGT3959200.022.639310-14
GGTCGCC3950200.022.42849250-54
GTGGTCG4024450.022.42753650-54
CGTCGTG4017800.022.2730210-14
Produced by FastQC (version 0.11.5)