FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454580705
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT83459721.8359714585774158Illumina Single End PCR Primer 1 (96% over 33bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC30590980.672949372103244Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA10644600.0125.2458651
ATCGGAA14230200.094.029552
GAGCGTC14202450.093.754749
TCGGAAG14428450.092.654233
AGAGCGT14579250.091.446138
CGGAAGA14733650.090.791724
AAGAGCG15063250.088.582447
GAAGAGC17791750.075.367816
GGAAGAG21410650.062.7087945
AGCGTCG13825800.034.601419
CGCCGTA13102950.027.40541555-59
TCGCCGT13279900.027.03042455-59
GCCGTAT13298200.026.87089755-59
GTACGTC13499400.026.56387930-34
GTCGCCG13466500.026.5434755-59
CGTATCA13469350.026.5037860-64
TACGTCC13563200.026.4953630-34
TGGTCGC13622900.026.27175150-54
GCGTCGT13779750.026.23142810-14
GTGTACG13732750.026.08880425-29
Produced by FastQC (version 0.11.5)