FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478424360
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG28520850.5961412583590016TruSeq Adapter, Index 8 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA7906450.063.5200081
TCGGAAG7984450.062.8299182
CGGAAGA8167800.061.3844873
AGAGCAC10363250.048.4491657
AGCACAC10532950.047.572649
GAGCACA10723050.046.816068
GAAGAGC11251350.044.7957955
AAGAGCA13688750.036.9820756
GGAAGAG14915400.034.0326044
TATGCCG4843800.021.06023645-49
CGTATGC5004250.020.1917245-49
CGCGCAT5069000.019.87142635-39
CTCGTAT4626200.019.72797440-44
ATGCCGT5160650.019.68739745-49
TCTCGCG5247400.019.39713330-34
TCTCGTA4695100.019.36620940-44
TCGCGCA5197800.019.35056735-39
GCCGTCT5211750.019.21092650-54
TGCCGTC5208400.019.10545245-49
GCGCATC5307600.019.08355135-39
Produced by FastQC (version 0.11.5)