FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471620306
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG10515810.2229719515088055TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG4797700.038.0352332
ATCGGAA4814050.037.9578631
CGGAAGA4999650.036.526063
AGAGCAC7182600.025.7010277
AGCACAC7303350.025.1967549
GAGCACA7506750.024.5796438
GAAGAGC8041400.023.0628495
AAGAGCA10571750.017.740716
TATGCCG2219950.016.79491445-49
GGAAGAG11548800.016.370154
CGCGAAA2362150.015.4527735-39
CGTATGC2418200.015.28432545-49
TCCGCGA2444500.015.10044530-34
CCGCGAA2424950.014.96867130-34
ATGCCGT2541650.014.75244145-49
TGCCGTC2553450.014.41172645-49
GCCGTCT2565450.014.37746950-54
CTCGTAT2396700.014.26328240-44
TCTCGTA2436500.014.02553340-44
TCGTATG2524100.013.34349640-44
Produced by FastQC (version 0.11.5)