FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423457863
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC6816760.1609784726089736Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1611000.065.9049769
GAGCGTC1952750.054.3976558
ATCGGAA2115700.050.9827581
TCGGAAG2251850.047.7113572
AGAGCGT2298100.046.405947
CGGAAGA2527650.042.5964163
AAGAGCG2729850.039.387756
GAAGAGC5376400.020.5301285
CGCCGTA1122400.019.55288155-59
TCGCCGT1247700.017.61369155-59
GCCGTAT1343550.016.49092555-59
GTCGCCG1368600.016.10645555-59
TGGTCGC1524600.014.680449550-54
CCGTATC1460400.014.62939655-59
CGTATCA1525150.014.5092260-64
GCGTCGT1535550.014.37495410-14
CGTCGTG1583050.013.99033510-14
GGTCGCC1550500.013.92746150-54
GTGGTCG1634050.013.7894250-54
GGAAGAG8810700.012.8899854
Produced by FastQC (version 0.11.5)