FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463429037
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG62539801.3495011103501484TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA8544100.0115.571781
TCGGAAG8626300.0114.40152
CGGAAGA9065350.0108.685563
AGAGCAC10923650.089.91927
AGCACAC11213050.087.4853749
GAGCACA11356800.086.420748
GAAGAGC11885200.082.8591165
AAGAGCA14144200.069.684956
GGAAGAG14710150.067.261544
CTCGTAT7557600.025.54157440-44
TCTCGTA7584900.025.42517740-44
TATGCCG7894750.025.12332345-49
ACTCCGG7960500.024.83810830-34
CGTATGC7877400.024.80578445-49
TCGTATG7749500.024.72724740-44
TCCGGAG8052750.024.58993530-34
CTCCGGA8071550.024.54415530-34
CCGGAGA8001450.024.53976830-34
ATCTCGT7951400.024.262240-44
ACACGTC8192100.024.14727610-14
Produced by FastQC (version 0.11.5)