FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463429037
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC61947841.3367276336635763Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG8230700.0117.695829
GAGCGTC8612750.0112.575678
ATCGGAA8861000.0110.58251
TCGGAAG8991450.0108.91772
AGAGCGT9008300.0107.8328867
CGGAAGA9432600.0103.674783
AAGAGCG9687500.0100.967756
GAAGAGC12488150.078.53385
GGAAGAG15912200.062.0098724
CGCCGTA7539700.025.47287255-59
GCCGTAT7754350.024.82365655-59
CCGTATC7800500.024.48999855-59
TGGTCGC7920900.024.48405650-54
TCGCCGT7820300.024.34215455-59
CGTATCA7811250.024.2714660-64
GGTCGCC8020550.024.16051550-54
GTGGTCG8087250.024.03439550-54
GTCGCCG8026300.023.97155455-59
TCTCGGT8223950.023.95577845-49
GCGTCGT8193200.023.72241410-14
Produced by FastQC (version 0.11.5)