FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493038856
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG4968510.10077319342149374TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1528250.053.947561
TCGGAAG1646050.050.2446062
CGGAAGA1986750.041.701113
AGAGCAC4663950.018.2074037
AGCACAC5003750.017.0375779
GAGCACA5164150.016.5322388
GAAGAGC5425750.015.8740035
TATGCCG1200500.014.34096745-49
CTCGTAT1221950.013.68850240-44
CGTATGC1270400.013.42638645-49
TCTCGTA1275150.013.17083840-44
TCGTATG1397650.012.19380640-44
ACCGCTC1439450.012.03353730-34
TATCTCG1396150.011.9411140-44
CGCTCAT1483150.011.78257330-34
GTCACCG1526000.011.48601725-29
ACACGTC1569300.011.105144510-14
TGCCGTC1607550.011.04240345-49
ATGCCGT1607000.010.94605945-49
GCCGTCT1585950.010.92907250-54
Produced by FastQC (version 0.11.5)