FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471270603
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG15628050.3316152100410133TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2820350.092.5293661
TCGGAAG3000150.087.013422
CGGAAGA3208400.081.239493
AGAGCAC5907800.044.4479457
AGCACAC6189200.042.413019
GAGCACA6335750.041.492618
GAAGAGC6431850.040.9606865
AAGAGCA9079800.029.3170556
GGAAGAG10143750.026.3566934
TATGCCG2205200.023.94367445-49
TCGTATC2350900.022.48753435-39
CTCGTAT2211600.022.28213340-44
CGTATGC2361400.022.22480245-49
TCTCGTA2242400.021.96832340-44
GTCACGA2478050.021.4476925-29
TATCTCG2302600.021.1767740-44
ATGCCGT2501800.021.16704445-49
TGCCGTC2506150.020.78785145-49
GCCGTCT2543150.020.48634750-54
ATTCGTA2594200.020.33883935-39
Produced by FastQC (version 0.11.5)