FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469880975
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG59908851.274979264695703TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA7357950.0126.161451
TCGGAAG7546900.0123.126142
CGGAAGA7907100.0117.3828053
AGAGCAC10338600.089.503577
AGCACAC10647250.086.8395169
GAGCACA10766700.085.907748
GAAGAGC11101700.083.4762045
AAGAGCA13608650.068.26146
GGAAGAG14555150.064.104374
CTCGTAT7036850.026.20978740-44
TATGCCG7198100.026.1703445-49
TCTCGTA7074050.026.04203840-44
GTCACGA7288300.025.84724625-29
TCGTATC7269500.025.83225335-39
CGTATGC7291800.025.48711645-49
TATCTCG7155700.025.3877140-44
TCGTATG7229150.025.2111840-44
AGTCACG7468550.025.21042825-29
ACACGTC7458350.025.19822510-14
GAATTCG7497750.025.169430-34
Produced by FastQC (version 0.11.5)