FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474945849
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG75927721.5986605664596514TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA10678000.0113.357111
TCGGAAG10934100.0110.980072
CGGAAGA11257300.0107.7261663
AGAGCAC13380750.090.500157
AGCACAC13621150.088.711619
GAGCACA13729450.088.1118858
GAAGAGC14295800.084.938085
AAGAGCA16717000.072.693766
GGAAGAG17825100.068.348384
TATGCCG9197950.026.5098645-49
CTCGTAT9030800.026.33580240-44
TCTCGTA9091550.026.1253840-44
CGTATGC9302650.025.89464245-49
TCGTATG9173750.025.73009340-44
TATCTCG9180300.025.56849940-44
ATGCCGT9562750.025.5217645-49
ATCTCGT9392350.025.27479440-44
TGCCGTC9628750.025.12833245-49
GCCGTCT9645700.024.92833550-54
CCGTCTT9820250.024.73372750-54
Produced by FastQC (version 0.11.5)