FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401397614
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG38691670.9639237666220904TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA6870800.093.317281
TCGGAAG6966200.092.103772
CGGAAGA7275550.088.113293
AGAGCAC9075050.070.661837
AGCACAC9228150.069.3159569
GAGCACA9356300.068.516828
GAAGAGC9850100.065.245025
AAGAGCA11986100.053.7714926
GGAAGAG12792600.050.5161824
TATGCCG5462150.023.85933945-49
CTCGTAT5291350.023.5185240-44
TCTCGTA5300250.023.29116240-44
CGTATGC5543250.023.26396245-49
ATGCCGT5735350.022.69602645-49
TACTCGA5666500.022.65513435-39
TTACTCG5631350.022.64681830-34
TCGTATG5429250.022.55008740-44
GCCGTCT5739050.022.3834250-54
ATCTCGT5544000.022.26556440-44
TGCCGTC5752350.022.24369845-49
Produced by FastQC (version 0.11.5)