FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401397614
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC38915020.969488074734794Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG6543250.096.754499
GAGCGTC6888200.091.901828
ATCGGAA7329200.087.73561
AGAGCGT7272200.087.277237
TCGGAAG7401750.086.669072
CGGAAGA7733150.082.917373
AAGAGCG7796450.081.882516
GAAGAGC10287600.062.269935
GGAAGAG13190750.048.9418374
CGCCGTA5200200.024.253855-59
GCCGTAT5422050.023.36363255-59
TCGCCGT5394400.023.29621155-59
TGGTCGC5537600.022.98330750-54
GTCGCCG5561800.022.7017255-59
CGTATCA5541500.022.66342560-64
CCGTATC5512200.022.61070655-59
GTGGTCG5676350.022.50660950-54
GGTCGCC5613000.022.32439850-54
GTACGTC5765500.021.97550830-34
TACGTCC5850900.021.82763930-34
Produced by FastQC (version 0.11.5)