FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439497763
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1416500.049.084241
TCGGAAG1553800.044.7026142
CGGAAGA1791200.038.7900163
AGAGCAC4347400.016.577417
TATGCCG897300.015.99925545-49
AGCACAC4647850.015.4887829
GAGCACA4715600.015.313778
GAAGAGC4808150.015.0759695
CGGCTAT955100.015.01893330-34
CGTATGC1020400.014.11885245-49
CTCGTAT941200.013.72185940-44
TCTCGTA990050.013.07263440-44
ACCGGCT1166400.012.38269130-34
ATGCCGT1192850.012.23205145-49
TGCCGTC1199650.011.692595545-49
GCCGTCT1235350.011.52871350-54
GTCACCG1279900.011.51781425-29
TCACCGG1234100.011.48357125-29
TCGTATG1105150.011.40677940-44
ATCTCGT1215800.010.83490840-44
Produced by FastQC (version 0.11.5)