FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439497763
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1415700.048.702589
GAGCGTC1798350.038.4650158
ATCGGAA1936600.036.412371
TCGGAAG2091450.033.681222
AGAGCGT2199450.031.7341827
CGGAAGA2367450.029.7648493
AAGAGCG2648650.026.5993046
CGCCGTA904550.015.73212155-59
GAAGAGC5308150.013.8241775
TCGCCGT1075550.013.34277255-59
GCCGTAT1140050.012.71759155-59
GTCGCCG1212000.011.86333655-59
CCGTATC1270450.011.18288455-59
CGTATCA1329150.010.97803960-64
TGGTCGC1379150.010.66419250-54
GCGTCGT1351650.010.58308410-14
CGTCGTG1409800.010.19386810-14
GGTCGCC1410550.010.14210450-54
GTGGTCG1489000.09.91736750-54
TCGTGTA1640100.08.95427710-14
Produced by FastQC (version 0.11.5)