FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477969143
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC17114410.3580651648886882Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3723100.072.216839
GAGCGTC4107250.065.530638
AGAGCGT4448350.060.679217
ATCGGAA4530600.059.9579931
TCGGAAG4646650.058.4154172
CGGAAGA4854800.055.8818133
AAGAGCG4972450.054.841036
GAAGAGC8123000.034.0322725
GGAAGAG12053350.023.3032764
CGCCGTA2485300.021.74735355-59
TCGCCGT2677150.020.13365455-59
GCCGTAT2726600.019.92058455-59
TGGTCGC2913100.018.77346450-54
CCGTATC2843800.018.75190755-59
GTCGCCG2915700.018.72906555-59
CGTATCA2904800.018.65605760-64
GGTCGCC2996200.017.93865250-54
GTGGTCG3099500.017.7043450-54
TCTCGGT3510600.015.97059745-49
TCGGTGG3528850.015.454432545-49
Produced by FastQC (version 0.11.5)