FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444077584
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG9764060.2198728409583493TruSeq Adapter, Index 3 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2076950.080.950011
TCGGAAG2215750.075.6715852
CGGAAGA2413900.069.410843
AGAGCAC4980200.033.8392567
AGCACAC5280550.031.910419
GAGCACA5366000.031.450888
GAAGAGC5505250.030.823235
TATGCCG1550100.022.1820545-49
GCGCATC1574400.021.69907635-39
AAGAGCA8020700.021.406296
TAATGCG1635950.020.99609230-34
CTCGTAT1561050.020.19394740-44
CGTATGC1692400.020.07285345-49
AATGCGC1682000.020.01260830-34
TGCGCAT1742250.019.65432435-39
TCTCGTA1600400.019.65304640-44
GGAAGAG8920950.019.4451834
ATGCGCA1754000.019.36977835-39
ATGCCGT1821900.018.9348845-49
CGCATCT1702650.018.5050135-39
Produced by FastQC (version 0.11.5)