FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444077584
Sequences flagged as poor quality0
Sequence length151
%GC40

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC10894270.2453235739095536Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1913100.087.783959
GAGCGTC2270800.074.066578
ATCGGAA2430950.070.387041
TCGGAAG2563900.066.511352
AGAGCGT2609600.064.636717
CGGAAGA2794700.060.9367263
AAGAGCG2998650.056.6593786
GAAGAGC5859550.029.5332535
CGCCGTA1460700.023.28339855-59
TCGCCGT1591550.021.43655255-59
GTCGCCG1702650.020.1535955-59
GCCGTAT1710200.020.01375655-59
GGAAGAG9300000.018.9781364
TGGTCGC1840950.018.79126750-54
GCGTCGT1850100.018.52704610-14
CCGTATC1825900.018.37555955-59
CGTCGTG1895000.018.1393210-14
GGTCGCC1875450.018.07298550-54
GTGGTCG1938500.017.93389750-54
CGTATCA1917050.017.88640260-64
Produced by FastQC (version 0.11.5)