FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465885862
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG8856650.19010342923864043TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3597650.045.468211
TCGGAAG3721200.043.7814062
CGGAAGA3926800.041.523083
AGAGCAC6415950.025.7569267
AGCACAC6701800.024.7729599
GAGCACA6858600.024.1505978
GAAGAGC7117600.023.3379765
AAGAGCA9590150.017.5544626
TATGCCG2045200.016.40809445-49
GGAAGAG10835400.015.695494
CGTATGC2200300.015.165174545-49
ATGCCGT2341900.014.38503145-49
CTCGTAT2038950.014.28502140-44
GCCGTCT2365550.014.02850250-54
TGCCGTC2348450.013.95293845-49
TCTCGTA2092200.013.93114340-44
TCGTATG2187150.013.0367140-44
CGTCTTC2643100.012.78079750-54
CCGTCTT2683950.012.57708950-54
ATCTCGT2348550.012.46052340-44
Produced by FastQC (version 0.11.5)