FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464555930
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG7088790.15259282127773077TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3033800.041.9723131
TCGGAAG3162700.040.2764662
CGGAAGA3380850.037.6779633
AGAGCAC5877400.022.0257077
AGCACAC6105550.021.1931659
GAGCACA6255850.020.7233728
GAAGAGC6518350.020.024475
TATGCCG1641050.015.94055445-49
AAGAGCA8982400.014.770156
CGTATGC1798050.014.46722245-49
CTCGTAT1647400.014.07762940-44
TCTCGTA1704700.013.59252940-44
ATGCCGT1954300.013.43445145-49
GGAAGAG10188350.013.1863264
GCCGTCT2002450.012.899250-54
TATCTCG1804350.012.75424640-44
TGCCGTC2006700.012.751965545-49
TCGTATG1808800.012.51122140-44
ATCTCGT1964800.011.92747540-44
CGTCTTC2286750.011.56941550-54
Produced by FastQC (version 0.11.5)