FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478841195
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC35085500.732716824833753Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG6785500.080.385719
GAGCGTC7248000.075.532928
AGAGCGT7622400.071.913237
ATCGGAA7910350.069.861861
TCGGAAG8007400.068.826212
CGGAAGA8176850.067.3701253
AAGAGCG8186800.067.292046
GAAGAGC11339750.048.886045
GGAAGAG15104650.036.9990654
CGCCGTA4537100.024.06177555-59
TCGCCGT4725550.022.98717755-59
GCCGTAT4774800.022.88426855-59
GTCGCCG4938150.022.27845855-59
CGTATCA4978400.021.84110360-64
TGGTCGC5067050.021.75503250-54
CCGTATC4930800.021.75483755-59
GGTCGCC5129500.021.1857550-54
GTGGTCG5232600.021.11772550-54
TCTCGGT5708500.019.58690545-49
TCGGTGG5753700.019.09382645-49
Produced by FastQC (version 0.11.5)