FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468512971
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG8773080.1872537270691701TruSeq Adapter, Index 3 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2088650.074.451
TCGGAAG2266250.068.660372
CGGAAGA2484450.062.65443
AGAGCAC5168900.030.498037
AGCACAC5478250.028.765149
GAGCACA5618000.028.0948648
GAAGAGC5743100.027.5346625
TATGCCG1477600.021.41954645-49
GCGCATC1509650.020.78383335-39
TAATGCG1577750.020.11599530-34
CGTATGC1616450.019.40208445-49
CTCGTAT1481650.019.3281540-44
AAGAGCA8358800.019.2547136
AATGCGC1630150.019.2052630-34
TGCGCAT1670700.018.82632435-39
TCTCGTA1519650.018.77901540-44
ATGCGCA1702100.018.45432335-39
ATGCCGT1771400.017.91687245-49
TGCCGTC1773600.017.5881545-49
CGCATCT1638150.017.41999835-39
Produced by FastQC (version 0.11.5)