FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476480933
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1569400.044.2295651
TCGGAAG1742700.039.6224822
CGGAAGA2048850.033.834643
AGAGCAC4721700.015.1744677
TATGCCG967850.015.02165245-49
AGCACAC5019850.014.2962369
GAGCACA5127650.014.090398
GAAGAGC5217750.013.8539865
CGTATGC1107300.013.04869245-49
CTCGTAT1039600.012.94231740-44
TCGTATC1131800.012.92703235-39
TCTCGTA1091800.012.38062940-44
GTCACGA1260800.011.66295925-29
ATGCCGT1271850.011.60555645-49
TATCTCG1188500.011.31597240-44
TGCCGTC1289950.011.02123945-49
GCCGTCT1334600.010.92561450-54
ATTCGTA1359200.010.78135135-39
TCGTATG1232550.010.66572840-44
GAATTCG1449550.010.25552930-34
Produced by FastQC (version 0.11.5)