FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476480933
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1941650.035.1410459
GAGCGTC2334950.029.3219268
ATCGGAA2520850.027.723121
TCGGAAG2678050.025.9172422
AGAGCGT2721350.025.2867287
CGGAAGA2948450.023.5661873
AAGAGCG3146150.022.0082826
CGCCGTA1192900.011.84938355-59
GAAGAGC6121350.011.8185325
TCGCCGT1356900.010.46235155-59
GCCGTAT1439400.010.01802155-59
GTCGCCG1527650.09.34607655-59
CGTATCA1636150.08.88910260-64
CCGTATC1583700.08.87143155-59
TGGTCGC1659200.08.73451150-54
GGTCGCC1719000.08.20289250-54
GTGGTCG1796300.08.13919650-54
CAGTACG1809950.07.624762530-34
GGAAGAG9942650.07.6229324
GCGTCGT1881000.07.574795210-14
Produced by FastQC (version 0.11.5)