FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392612560
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG37223250.9480911665179534TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG8796450.069.8611152
ATCGGAA8861950.069.2723541
CGGAAGA9115050.067.400873
AGAGCAC10425150.058.9378437
AGCACAC10474150.058.50719
GAGCACA10619800.057.8227588
GAAGAGC11458600.053.822155
AAGAGCA13360350.046.2927976
GGAAGAG14125500.043.8896334
TATGCCG5701950.021.6936945-49
CTCGTAT5515950.021.15135240-44
CGTATGC5786100.021.09927445-49
TCTCGTA5601250.020.82070240-44
TCGTATG5640650.020.44983940-44
ATGCCGT6108150.020.25530445-49
CCGTCTT6218450.019.89462550-54
CGTCTTC6247750.019.84171150-54
TGCCGTC6188750.019.79024945-49
ATCTCGT5899350.019.75853540-44
CATCTCG5992550.019.25549940-44
Produced by FastQC (version 0.11.5)