FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397938954
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG35516900.8925213187347323TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA5893100.099.349811
TCGGAAG6005100.097.632412
CGGAAGA6237500.093.9295963
AGAGCAC8129650.071.581557
AGCACAC8336350.069.7375269
GAGCACA8453700.068.821748
GAAGAGC8836000.066.184985
AAGAGCA10953600.053.4235536
GGAAGAG11845350.049.8772934
TATGCCG4773900.024.70256445-49
CTCGTAT4690750.024.37856540-44
TCTCGTA4744450.024.20562440-44
CGTATGC4857050.023.99637845-49
TCGTATC4948800.023.7735135-39
TATCTCG4837500.023.5155340-44
TCGTATG4857900.023.16636740-44
GTCACGA5123600.023.14923725-29
ATGCCGT5089900.023.12478345-49
ATCTCGT4988450.023.07862340-44
TGCCGTC5086950.022.70239345-49
Produced by FastQC (version 0.11.5)