FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397938954
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC35286520.8867319885451577Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG5773850.0100.635029
GAGCGTC6098100.095.156578
ATCGGAA6437950.091.492131
AGAGCGT6390800.090.56137
TCGGAAG6511200.090.0297242
CGGAAGA6726600.086.900653
AAGAGCG6796800.085.537256
GAAGAGC9319650.062.7314385
GGAAGAG12403150.047.4564744
CGCCGTA4688600.024.60583755-59
TCGCCGT4853300.023.58562955-59
GCCGTAT4905250.023.55305955-59
TGGTCGC5009650.023.12041350-54
GTCGCCG4983200.023.04266255-59
CGTATCA5019350.022.87482560-64
CCGTATC4974400.022.83600455-59
GGTCGCC5041550.022.66989550-54
GTGGTCG5178450.022.394250-54
TCTCGGT5430700.021.40436445-49
CTCGGTG5561050.020.90108545-49
Produced by FastQC (version 0.11.5)