FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457389295
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG6668290.1457902507316005TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2618750.041.9437181
TCGGAAG2725050.040.379552
CGGAAGA2957000.037.2178463
AGAGCAC5342450.021.0580947
AGCACAC5615850.019.9737059
GAGCACA5764700.019.5131578
GAAGAGC6044250.018.6574065
TATGCCG1302850.017.38054545-49
CGTATGC1436100.015.63965445-49
CTCGTAT1390900.015.54431740-44
TCTCGTA1451700.014.8952940-44
ATGCCGT1594500.014.34786745-49
TGCCGTC1605950.013.96569645-49
TCGTATG1544300.013.85473140-44
GCCGTCT1650450.013.69275850-54
AAGAGCA8578700.013.4250586
GTCACGA1708100.013.27225525-29
ATCTCGT1699850.012.82829540-44
ACGAGAT1848400.012.36689330-34
GGAAGAG9634150.012.0995274
Produced by FastQC (version 0.11.5)