FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457389295
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC6966350.15230680027174662Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2165900.050.5850229
GAGCGTC2550700.043.093918
AGAGCGT2889400.038.125137
ATCGGAA2970700.037.3829571
TCGGAAG3079550.036.0627822
CGGAAGA3311650.033.5484473
AAGAGCG3381700.032.8149456
CGCCGTA1178450.018.86742455-59
GAAGAGC6437350.017.7697875
TCGCCGT1308300.017.03579755-59
GCCGTAT1409450.015.98183355-59
GTCGCCG1412550.015.7159355-59
TGGTCGC1529550.014.75395550-54
CCGTATC1546250.014.38888255-59
GGTCGCC1583100.014.03884850-54
GTGGTCG1620500.013.93483650-54
CGTATCA1616650.013.92811960-64
GGAAGAG10081000.011.7180034
TCTCGGT1998600.011.51161345-49
TCGGTGG2039550.011.16395545-49
Produced by FastQC (version 0.11.5)