FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384117135
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC16522560.430143789341759Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG4980150.051.0999039
GAGCGTC5361250.047.461988
AGAGCGT5680450.044.9244047
ATCGGAA5912150.043.6240121
TCGGAAG5996700.042.9142232
CGGAAGA6091550.042.1923943
AAGAGCG6123200.041.852756
GAAGAGC8566350.030.23785
GGAAGAG11692100.022.4233194
CGCCGTA2714400.018.98930455-59
GCCGTAT2898500.017.86121455-59
TCGCCGT2897250.017.78535355-59
CGTATCA3004950.017.11948460-64
CCGTATC2990300.016.90515355-59
GTCGCCG3078650.016.81463655-59
TGGTCGC3197600.016.30784450-54
GGTCGCC3246550.015.74935850-54
GTGGTCG3372900.015.50325850-54
TCTCGGT3839750.013.78520545-49
TCGGTGG3843700.013.436419545-49
Produced by FastQC (version 0.11.5)