FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459219529
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG21586420.47006755237536946TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3199950.0106.496151
TCGGAAG3403150.0100.480762
CGGAAGA3633150.094.0811163
AGAGCAC6379200.053.656597
AGCACAC6714600.050.9512759
GAGCACA6832650.050.1480878
GAAGAGC6884750.049.9407965
AAGAGCA9521150.036.2515226
GGAAGAG10402550.033.4091844
TATGCCG2869950.024.15247245-49
CTCGTAT2932000.023.18290540-44
TCCGATC379700.023.1481113
TTACTCG3055400.022.90837330-34
TCTCGTA2983000.022.81036240-44
CGTATGC3028400.022.63317545-49
TCGTATG3068800.022.39083540-44
TACTCGA3082000.022.2177535-39
TGCCGTC3158900.022.20064445-49
ATGCCGT3147350.022.11722645-49
GCCGTCT3207350.021.39804850-54
Produced by FastQC (version 0.11.5)