FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459219529
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC21908930.4770905550926603Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT2271100.0103.4566042
AGCGTCG3418100.099.078879
CTTCCGA2426700.097.015971
GAGCGTC3784500.089.515328
ATCGGAA3851950.089.089991
TCGGAAG4047000.084.812
AGAGCGT4116450.082.496427
CGGAAGA4274650.080.189313
AAGAGCG4579100.074.650796
TCCGATC3197650.073.39593
CCGATCT3336100.069.513384
CGATCTA1152100.063.2627075
GAAGAGC7548350.045.684035
CGATCTG1055750.036.8559275
CGATCTT1884500.031.8024235
GGAAGAG11326550.030.83954
CGCCGTA2844050.024.205555-59
TCGCCGT2956350.023.18696855-59
GCCGTAT3094100.022.33273355-59
GTCGCCG3058000.022.30338155-59
Produced by FastQC (version 0.11.5)