FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461707081
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG5922950.12828371588262472TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1802050.059.18771
TCGGAAG1942400.054.9782682
CGGAAGA2157400.049.435433
AGAGCAC4762650.022.9082937
CGCGAAA944650.022.19632335-39
TCCGCGA984650.021.51807830-34
AGCACAC5096150.021.4200089
GAGCACA5201750.021.0534428
CCGCGAA1009100.020.75240930-34
GAAGAGC5322450.020.6404975
TATGCCG1129350.019.23654445-49
CGTATGC1262800.017.10953145-49
CTCGTAT1158550.016.80836740-44
CTCCGCG1277650.016.76499630-34
GCGAAAT1286250.016.74103735-39
TCTCGTA1202950.016.13375340-44
ACTCCGC1403800.015.46710430-34
ATGCCGT1417850.015.4194845-49
TGCCGTC1415750.015.24677545-49
GCCGTCT1456650.014.783195550-54
Produced by FastQC (version 0.11.5)