FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493832680
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC9258650.18748556697381794Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2219150.066.926299
GAGCGTC2573400.057.8526768
ATCGGAA2857000.052.9097941
AGAGCGT2943800.050.807317
TCGGAAG3027100.050.0195582
CGGAAGA3248900.046.5860523
AAGAGCG3509350.042.999426
TCCGATC494100.030.0002021
GAAGAGC6779650.022.7046075
CCGATCT652650.022.420362
CGCCGTA1607900.018.85995155-59
TCGCCGT1711150.017.68467955-59
TTCCGAT636800.017.4016742
GTCGCCG1824700.016.6564655-59
GCCGTAT1882600.016.25428655-59
TGGTCGC1988500.015.49512450-54
CCGTATC1989250.015.07971855-59
GGTCGCC2028450.014.96055750-54
CGTATCA2067450.014.721621560-64
GTGGTCG2134450.014.50622450-54
Produced by FastQC (version 0.11.5)