FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471903691
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG29047500.6155387328809853TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA4471950.0105.6546551
TCGGAAG4646700.0101.6053542
CGGAAGA4852100.097.185423
AGAGCAC7518250.062.8820157
AGCACAC7848850.060.1850629
GAGCACA7982900.059.1928638
GAAGAGC8129250.058.2832765
AAGAGCA10738450.044.3222546
GGAAGAG11846900.040.2903984
TATGCCG3713750.025.68102845-49
CTCGTAT3635450.024.82805840-44
CGTATGC3851400.024.48986245-49
TCTCGTA3689450.024.43715940-44
ATGCCGT4010350.023.7574745-49
TCGTATG3796200.023.51278940-44
TGCCGTC4013300.023.43761345-49
GCCGTCT4056400.023.152450-54
ATCTCGT3924350.023.01801940-44
CCGGAGA4187400.022.55975530-34
ACTCCGG4231150.022.55675930-34
Produced by FastQC (version 0.11.5)