FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463750346
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1127750.032.7936971
TCGGAAG1292450.028.7587992
CGGAAGA1525750.024.3613383
CGCGCAT484200.014.74291535-39
TCGCGCA509250.014.564335-39
TCTCGCG554600.013.59597430-34
TATGCCG651650.011.74835645-49
GCGCATC677700.010.76448535-39
CTCGCGC744200.010.09316330-34
CGTATGC783100.09.89847445-49
AGAGCAC4177900.09.5782877
AGCACAC4510950.08.8518479
GAGCACA4609000.08.6886988
CTCGTAT706950.08.56755340-44
ACTCTCG937350.08.46654730-34
GAAGAGC4739500.08.4435875
ATGCCGT959600.08.22589145-49
TGCCGTC953500.08.0185345-49
TCTCGTA756600.07.86737140-44
GCCGTCT995700.07.738419550-54
Produced by FastQC (version 0.11.5)