FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463750346
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1130450.032.450019
GAGCGTC1514850.024.464488
ATCGGAA1675450.022.7240431
TCGGAAG1835450.020.7293112
AGAGCGT1886600.019.8474357
CGGAAGA2088250.018.2293663
AAGAGCG2347550.016.184676
CGCCGTA680700.011.26566455-59
TCGCCGT823200.09.47580155-59
GCCGTAT932650.08.45239255-59
GTCGCCG971750.08.06305855-59
GAAGAGC5256050.07.82302765
CCGTATC1057550.07.371877755-59
GCGTCGT1038850.07.320322510-14
TGGTCGC1117150.07.19432350-54
CGTATCA1145200.07.042852460-64
CGTCGTG1100550.06.96919710-14
GGTCGCC1146850.06.855058750-54
GTGGTCG1242000.06.566838350-54
TCGTGTA1392200.05.754964410-14
Produced by FastQC (version 0.11.5)