FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413637828
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC6565940.1587364490270943Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3076100.032.4149789
GAGCGTC3443050.029.0296788
AGAGCGT3819800.026.2400637
ATCGGAA4075600.024.8393381
TCGGAAG4092350.024.6986982
AAGAGCG4261400.023.6707976
CGGAAGA4286050.023.6694643
CGCCGTA1350150.015.13493155-59
GAAGAGC6948150.014.9568955
TCGCCGT1525250.013.45445855-59
GCCGTAT1572650.013.14481255-59
GTCGCCG1593600.012.90923455-59
TGGTCGC1710900.012.19291250-54
CCGTATC1678000.012.07848855-59
CGTATCA1716200.012.02342260-64
GGTCGCC1727650.011.795258550-54
GTGGTCG1817550.011.51973350-54
GGAAGAG10044850.010.6515254
TCTCGGT2212650.09.64641845-49
CCGTGTA2186800.09.63971735-39
Produced by FastQC (version 0.11.5)