FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416538503
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG32953490.7911271050013833TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG7676000.069.997232
ATCGGAA7705950.069.648381
CGGAAGA7998100.067.232663
AGAGCAC9557450.056.3246237
AGCACAC9611600.055.9017149
GAGCACA9771050.055.068128
GAAGAGC10620850.050.8462645
AAGAGCA12647600.042.771056
GGAAGAG13550450.040.1052864
TATGCCG4934300.021.96005845-49
CTCGTAT4822250.021.56653440-44
CGTATGC5022100.021.33101545-49
TCTCGTA4886600.021.27600340-44
TCGTATG4959400.020.6631440-44
ATGCCGT5335850.020.39603845-49
ATCTCGT5148150.020.25253940-44
TGCCGTC5391250.019.91965345-49
CGTCTTC5500050.019.78590450-54
CCGTCTT5506600.019.70891850-54
CCGGAGA5497700.019.51442730-34
Produced by FastQC (version 0.11.5)