FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336724451
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG35184911.0449169905989393TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA5060650.0116.518231
TCGGAAG5192850.0113.5096052
CGGAAGA5342250.0110.275153
AGAGCAC7187000.082.070377
AGCACAC7437900.079.2377859
GAGCACA7496450.078.639038
GAAGAGC7604250.077.6621255
AAGAGCA9467050.062.5204286
GGAAGAG10187900.058.2359434
TATGCCG4455850.026.4726145-49
CTCGTAT4259750.025.89260940-44
TCGTATC4584750.025.76615135-39
CGTATGC4559950.025.64473545-49
TCTCGTA4298600.025.63228840-44
ATGCCGT4625750.025.2482945-49
GTCACGA4716050.025.13995725-29
TATCTCG4345700.025.11458640-44
TGCCGTC4631150.024.85286945-49
GCCGTCT4636250.024.81726650-54
TCGTATG4392650.024.76924540-44
Produced by FastQC (version 0.11.5)