FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472184366
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1597050.032.7180069
GAGCGTC1967600.026.7139028
ATCGGAA2121900.025.1586631
TCGGAAG2288250.023.3381712
AGAGCGT2344100.022.5710897
CGGAAGA2513700.021.2561363
AAGAGCG2779750.019.226686
CGCCGTA978150.011.07577855-59
GAAGAGC5803350.09.813955
TCGCCGT1123100.09.66696755-59
GCCGTAT1236400.08.98985555-59
GTCGCCG1287800.08.53646755-59
CCGTATC1361450.07.99373455-59
CGTATCA1428700.07.862817360-64
TGGTCGC1426700.07.837222650-54
GGTCGCC1476150.07.417529650-54
GTGGTCG1562850.07.218482550-54
GCGTCGT1544550.07.013949410-14
CAGTACG1567150.06.870225430-34
CGTCGTG1599750.06.816339510-14
Produced by FastQC (version 0.11.5)