FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459811634
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC5155350.1121187377351135Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2483600.032.093139
GAGCGTC2894900.027.663618
ATCGGAA3218650.025.3069231
GATCGGA2440850.024.9670541
AGAGCGT3266100.024.6922727
TCGGAAG3314350.024.5808852
CGGAAGA3575100.022.7643433
AAGAGCG3777550.021.5589666
CGCCGTA1516100.018.49665655-59
TCGCCGT1706600.016.50503355-59
GCCGTAT1752550.016.09793755-59
GTCGCCG1878050.015.01293355-59
TGGTCGC1962200.014.44915350-54
CGTATCA1963700.014.37336760-64
GTGGTCG2116100.013.49148550-54
GAAGAGC6667650.012.718565
TCTCGGT2448000.011.82996645-49
GCGTCGT2391600.011.71639210-14
CGTCGTG2429350.011.56416510-14
CTCGGTG2654750.010.95125445-49
Produced by FastQC (version 0.11.5)