FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449262345
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG15410670.3430216258164258TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA4830150.054.7213441
TCGGAAG4938750.053.5462882
CGGAAGA5139850.051.4493143
AGAGCAC7522250.035.3361747
AGCACAC7715750.034.384739
GAGCACA7887300.033.733788
GAAGAGC8093900.033.0061455
AAGAGCA10508050.025.5977346
GGAAGAG11618550.023.3753954
TATGCCG2916650.018.6641945-49
CGTATGC3067900.017.59140645-49
CTCGTAT2892400.017.27757540-44
ATGCCGT3219150.016.91213845-49
TCTCGTA2971350.016.8121640-44
GCCGTCT3255250.016.50209850-54
TGCCGTC3244950.016.30323845-49
TCGTATG3065850.015.87785540-44
CGTCTTC3467500.015.71398550-54
ATCTCGT3229900.015.509940-44
CCGTCTT3523750.015.46067250-54
Produced by FastQC (version 0.11.5)