FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449820127
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3508050.018.5896669
GAGCGTC3961950.016.5092548
AGAGCGT4360400.015.0537637
ATCGGAA4502650.014.7771331
TCGGAAG4596550.014.4258182
CGGAAGA4886350.013.5796773
AAGAGCG5002850.013.2762816
CGCCGTA1355200.010.25969555-59
GAAGAGC7661950.09.0940945
GCCGTAT1552550.09.07592855-59
TCGCCGT1591000.08.82926955-59
CGTATCA1711300.08.20688760-64
CCGTATC1684450.07.946377355-59
GTCGCCG1824450.07.72809555-59
TGGTCGC1914750.07.41351250-54
GTGGTCG2082100.06.865658850-54
GGTCGCC1995750.06.782329650-54
GGAAGAG11242500.06.50882344
TCTCGGT2506700.05.89370245-49
GCGTCGG653400.05.661676145
Produced by FastQC (version 0.11.5)