FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464498361
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC8263820.1779084856663251Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2261350.056.3377159
GAGCGTC2629250.048.58978
ATCGGAA2908400.044.800791
TCGGAAG3060400.042.4875682
AGAGCGT3028700.042.3827637
CGGAAGA3299950.039.403623
AAGAGCG3558500.036.416876
GAAGAGC6509450.020.3707455
CGCCGTA1555800.016.63121655-59
TCGCCGT1706600.015.18627155-59
GCCGTAT1806300.014.47728155-59
GTCGCCG1869800.013.89412455-59
CCGTATC1909950.013.50640555-59
TGGTCGC1990950.013.18240650-54
GGAAGAG10382350.013.1775864
CGTATCA2008150.013.01549760-64
GGTCGCC2047650.012.6920750-54
GTGGTCG2162950.012.22863250-54
GCGTCGT2141650.012.07461810-14
CGTCGTG2203700.011.76423410-14
Produced by FastQC (version 0.11.5)