FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484365804
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG20175860.41654179203782105TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3681150.095.434121
TCGGAAG3872650.090.815152
CGGAAGA4078500.086.128313
AGAGCAC6835700.051.6621977
AGCACAC7172450.049.1840869
GAGCACA7306800.048.386878
GAAGAGC7433400.047.6990175
AAGAGCA10111200.035.3026166
GGAAGAG11262950.031.8322324
TATGCCG2881350.024.77122945-49
CTCGTAT2825750.023.3514840-44
CGTATGC3034100.023.19636345-49
TCTCGTA2869100.023.00471940-44
ATGCCGT3167700.022.49583445-49
CGCTCAT3140700.022.47943330-34
TATCTCG2930700.022.1981740-44
TGCCGTC3194500.021.9762845-49
ACCGCTC3216200.021.93820630-34
TCGTATG3002200.021.68640140-44
GCCGTCT3234450.021.62613950-54
Produced by FastQC (version 0.11.5)