FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484365804
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC22339390.4612090658654342Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3440600.0102.10839
GAGCGTC3838250.091.623078
ATCGGAA4036600.087.922221
AGAGCGT4145900.084.859087
TCGGAAG4218750.084.1678162
CGGAAGA4448600.079.68863
AAGAGCG4600200.076.842666
GAAGAGC7863000.045.374325
GGAAGAG11757550.030.7029554
CGCCGTA2782550.025.40036255-59
TCGCCGT2912500.024.23120955-59
GCCGTAT3036850.023.40704755-59
GTCGCCG3057300.023.05132555-59
GTACGTC3135150.022.57832730-34
TGGTCGC3142000.022.50197850-54
CCGTATC3164400.022.12591755-59
TACGTCC3244100.021.92463130-34
CGTATCA3241500.021.72159460-64
GGTCGCC3224250.021.69865250-54
GTGGTCG3280000.021.59149250-54
Produced by FastQC (version 0.11.5)