FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468874274
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG18202340.3882136642881797TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3319700.095.050751
TCGGAAG3519850.089.419932
CGGAAGA3773250.083.3848653
AGAGCAC6466050.048.9236687
AGCACAC6818150.046.3828479
GAGCACA6878250.046.023938
GAAGAGC6969650.045.4904445
AAGAGCA9511700.033.598816
GATCGGA990400.030.5066571
GGAAGAG10710950.029.9708924
TATGCCG2816950.024.4376345-49
CTCGTAT2720100.023.1641740-44
TCGTATC2979600.023.13795335-39
CGTATGC2967200.023.05652245-49
TCTCGTA2760900.022.814540-44
GTCACGA3108200.022.22104825-29
ATGCCGT3092100.022.14582845-49
TATCTCG2869400.021.79517740-44
ATTCGTA3185850.021.5030235-39
GCCGTCT3138650.021.48783350-54
Produced by FastQC (version 0.11.5)