FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468874274
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC20291910.4327793424639886Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3954400.080.559989
GAGCGTC4347450.073.3350148
ATCGGAA4554950.071.1391751
TCGGAAG4750350.067.908142
AGAGCGT4790000.066.663877
CGGAAGA4989100.064.5409853
AAGAGCG5318550.060.3230676
GAAGAGC8195950.039.5115935
GGAAGAG11978250.027.4092544
CGCCGTA3245200.021.49197855-59
TCGCCGT3400200.020.5152455-59
GCCGTAT3482000.020.10325255-59
GTCGCCG3597100.019.40879255-59
CGTATCA3662300.019.01163360-64
TGGTCGC3730200.018.82762150-54
GCGTCGT3831150.018.21186610-14
GTGGTCG3879150.018.1850550-54
CGTCGTG3904300.017.91483510-14
CCGTATC3593900.017.77872355-59
TCGTGTA4117150.017.05133410-14
Produced by FastQC (version 0.11.5)